CD97 antibody

Catalog No. ABIN119250

This anti-CD97 antibody is a Mouse Monoclonal antibody detecting CD97 in IP and FACS. Suitable for Human.

Quick Overview for CD97 antibody (ABIN119250)

Target: CD97

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD97 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: MEM-180

Product Details anti-CD97 Antibody

Specificity: The antibody recognizes an unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: PHA-activated blood cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/200 - 1/400, use 10 μL of the suggested working dilution to label 10^6 cells. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD97

Target: CD97

Alternative Name: CD97

Background: CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.Synonyms: Leukocyte antigen CD97

Gene ID: 976

NCBI Accession: NP_001020331

UniProt: P48960